An opsonised electrode. The direct electrochemical detection of superoxide generated by human neutrophils

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Abstract

A pyrolytic graphite electrode was surface modified with human IgG and used as a stimulus to elicit a respiratory burst from human neutrophils. The oxidation current observed was shown to be due to re-oxidation of superoxide produced by the neutrophils. Both superoxide dismutase and N-ethylmaleimide were effective inhibitors of the oxidation current. © 1984.

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Green, M. J., Hill, H. A. O., Tew, D. G., & Walton, N. J. (1984). An opsonised electrode. The direct electrochemical detection of superoxide generated by human neutrophils. FEBS Letters, 170(1), 69–72. https://doi.org/10.1016/0014-5793(84)81370-8

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