Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II)

  • Moritz A
  • Grzeschik K
  • Wingender E
 et al. 
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Abstract

A complete cDNA encoding the acrosin-trypsin inhibitor, HUSI-II, was used as a probe to isolate genomic clones from a human placenta library. Three clones which cover the entire HUSI-II gene were isolated and characterized. The exon-intron organization of the gene was determined and found to be identical to other known Kazal-type inhibitor-encoding genes. The striking similarity in the amino acid sequences which was found previously in HUSI-II and glycoprotein hormone β-subunits, is neither reflected in codon usage nor in the exon-intron arrangement of the genes. A 1.8-kb segment 5′ of the gene was sequenced. The analysis of this sequence showed that HUSI-II contains a G + C-rich region upstream from the transcription start point (tsp) which fulfills the criteria for a CpG island. Furthermore, in the first intron, a potential glucocorticoid-responsive element was found as a half-palindrome flanked by two CACCC elements. Determination of the tsp by S1 mapping revealed that HUSI-II has multiple tsp. Genomic Southern hybridization was used to show that HUSI-II is a single-copy gene. The localization of the gene to chromosome 4 was determined by hybridization of a 5′ genomic fragment to the DNA of a panel of somatic hybrids between human and rodent cells. © 1993.

Author-supplied keywords

  • Kazal-type inhibitor
  • acrosin inhibitor
  • chorionic gonadotropin
  • chromosomal localization
  • exon-intron mapping
  • glycoprotein hormones
  • nucleotide sequence analysis
  • transcription start point

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Authors

  • Annemarie Moritz

  • Karl Heinz Grzeschik

  • Edgar Wingender

  • Edwin Fink

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