Oxygen regulates brain angiogenesis and tight junction formation in blood–brain barrier

  • Lee S
  • Kim W
  • Choi Y
 et al. 
  • 6

    Readers

    Mendeley users who have this article in their library.
  • 0

    Citations

    Citations of this article.

Abstract

The maintenance of the blood–brain barrier (BBB) is important in the central nervous system because disruption of the BBB may contribute to many brain disorders, including Alzheimer's disease and stroke. However, the molecular mechanism of brain vessel development and BBB formation remains ill-defined. We found that the immunoreactivities of pimonidazol, hypoxic marker and vascular endothelial growth factor (VEGF) were detected in developing brain, and then disappeared after birth. However, the expression of occludin was gradually increased during BBB maturation. Moreover, we found src-suppressed C-kinase substrate (SSeCKS)-overexpressed in astrocytes during reoxygenation. Overexpressed SSeCKS strongly reduced the VEGF expression and angiogenesis. Furthermore, SSeCKS overexpression increased the expression of both angiopoietin-1 (Ang-1), an antipermeability factor, and tight junction proteins, consequently decreased [3H]sucrose permeability. We therefore conclude that reoxygenation of astrocytes may act as a significant driving force for the BBB maturation, and SSeCKS is a novel regulator of BBB differentiation by modulating both brain angiogenesis and tight junction formation. © 2004, Elsevier Inc. All rights reserved.

Author-supplied keywords

  • Angiogenesis
  • Blood–brain barrier
  • Oxygen tension
  • SSeCKS

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free