Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter through a highly conserved region

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Abstract

Xenopus Zic3 (zinc finger in the cerebellum-3) is expressed in the dorsal neural tube of tailbud embryos and tadpoles. We have isolated a 3.1 kb DNA fragment from the Xenopus laevis Zic3 locus that drives proper expression of a GFP reporter in transgenic frog tailbud embryos and tadpoles. This fragment contains regions that are highly similar among frogs, mice, and humans. One extremely conserved region contains a predicted Pbx binding site. We found that the transcription factors Pbx1b and Meis1 can bind this site and synergistically transactivate expression of a reporter containing the conserved region. Finally, we found that an intact Pbx site is essential for normal Zic3 promoter activity in transgenic frog embryos. Our data strongly suggest that a highly conserved region of the Zic3 promoter functions by direct interaction with Pbx1b and Meis1. © 2006 Elsevier Inc. All rights reserved.

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Kelly, L. E., Carrel, T. L., Herman, G. E., & El-Hodiri, H. M. (2006). Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter through a highly conserved region. Biochemical and Biophysical Research Communications, 344(3), 1031–1037. https://doi.org/10.1016/j.bbrc.2006.03.235

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