Intermediate sized filaments reconstituted in vitro from purified desmin, epidermal keratin and the Mr68,000 protein of neurofilaments were examined after high resolution metal shadowing. The filaments demonstrate a marked longitudinal periodicity of about 21 nm. This is the first procedure that allows detection of a periodic substructure in these filaments using the electron microscope. © 1982.
Henderson, D., Geisler, N., & Weber, K. (1982). A periodic ultrastructure in intermediate filaments. Journal of Molecular Biology, 155(2), 173–176. https://doi.org/10.1016/0022-2836(82)90444-2