The subcellular distribution of phosphatidylinositol(PI) kinase (EC 18.104.22.168), PI-specific phospholipase C (EC 22.214.171.124) and D, diacylglycerol (DG) kinase (EC 126.96.36.199), CTP:phosphatidic acid (PA) cytidyltransferase (EC 188.8.131.52) and PI-synthase (EC 184.108.40.206) in membrane fractions from suspension cultured Catharanthus roseus cells was investigated. Whereas activities of phospholipase C and D could be detected both in the cytosol and membrane fractions, all the other enzymes were bound to membranes. PI-kinase, membrane-bound phospholipase C and D, DG-kinase and PI-synthase had their highest specific activities in the plasma membrane enriched fraction, whereas cytidyltransferase had comparable activities both in the plasma membrane fraction and in the fractions derived from internal membranes. These data suggest that the plant plasma membrane might be autonomous with respect of the recycling of lipid cleavage products obtained by the action of phospholipase C and D. © 1992.
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