Potentiometric measurement of lipoic acid reduction in microbial cultures. Part I. Influence of bacterial growth on the potential-time signals

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Abstract

In this paper, we investigate the theoretical relations between microbial growth and the time evolution of the zero-current potential of a gold electrode in microbial cultures provided with exogenic lipoic acid. A linear relationship was elicited by computer calculation between simple potential versus time data and inverses of apparent growth rates of organisms. To verify this relation experimentally, we carried out potential-time measurements in cultures supplied with increasing concentrations of different sugars, and followed the kinetics of bacterial growth by classical photometric measurements. Judging from the theory, the potentiometric and growth photometric data were in good agreement. These results show that the potentiometric data may be used as reliable indexes for bacterial growth. © 1984.

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Junter, G. A. (1984). Potentiometric measurement of lipoic acid reduction in microbial cultures. Part I. Influence of bacterial growth on the potential-time signals. Bioelectrochemistry and Bioenergetics, 12(1–2), 81–92. https://doi.org/10.1016/0302-4598(84)85152-1

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