5-, 8-, 9-, 11-, 12- and 15 hydroperoxy-eicosatetraenoic acids (HPETEs) were generated from arachidonic acid by a reaction with H2O2and Cu++ ions. They were purified by high performance liquid chromatography, either on a silica gel (μ Porasil) column or on a reversed phase (μ Bondapak C18) column. The yield of 5-HPETE was considerably greater when the μ Bondapak C18column was used. The HPETEs were characterized and assayed by their ability to oxidize triphenylphosphine: triphenylphosphine oxide formation was monitored by gas chromatography. When stored in methylene chloride at -20°C, the HPETEs were stable for several months. © 1980.
Boeynaems, J. M., Oates, J. A., & Hubbard, W. C. (1980). Preparation and characterization of hydroperoxy-eicosatetraenoic acids (HPETEs). Prostaglandins, 19(1), 87–97. https://doi.org/10.1016/0090-6980(80)90156-2