Preparation of Photosynthetically Active Particles from Synchronized Cultures of Unicellular Algae

  • Senger H
  • Mell V
  • 2

    Readers

    Mendeley users who have this article in their library.
  • 58

    Citations

    Citations of this article.

Abstract

This chapter describes the methods for the preparation of photosynthetically active particles from synchronized cultures of unicellular algae. The preparation of chloroplasts and chloroplast particles from higher plants provides homogeneous experimental material than the whole cells, abolishes the effects of the stomatal barrier, transport, and permeation problems. The chapter establishes a method for (1) fast disruption with high yield, (2) high activity of the particles, (3)applicability at different stages of synchronous cultures of different algae, and (4) high reproducibility. A comparative characterization of the photosynthetic behavior and the changes in the photosynthetic apparatus of synchronized cultures of Chlorella, Scenedesmus, and Chlamydomonas has been reported. The particle characterization includes size and pigment analysis. The effectiveness of the procedure in the preparation of photosynthetically active particles can be evaluated only in photosynthetic experiments. High reproducibility of experimental results is most desirable. Synchronous cultures are standardized and present therefore a good initial experimental material.© 1977 Academic Press Inc.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Horst Senger

  • Volker Mell

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free