Preparative purification of Escherichia coli heat-stable enterotoxin

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Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STawhich exhibited biochemical homology to STapurified by more complicated procedures. © 1985.




Saeed, A. M. K., & Greenberg, R. N. (1985). Preparative purification of Escherichia coli heat-stable enterotoxin. Analytical Biochemistry, 151(2), 431–437.

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