Preparative purification of Escherichia coli heat-stable enterotoxin

  • Saeed A
  • Greenberg R
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Abstract

Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STa which exhibited biochemical homology to STa purified by more complicated procedures.

Author-supplied keywords

  • Amino Acids/analysis
  • Animals
  • Bacterial Toxins/*isolation & purification
  • Biological Assay
  • Cattle
  • Chromatography, Gel/methods
  • Chromatography, High Pressure Liquid/methods
  • Chromatography, Ion Exchange/methods
  • Enterotoxins/*isolation & purification
  • Escherichia coli/analysis
  • Mice
  • Species Specificity

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Authors

  • A M Saeed

  • R N Greenberg

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