Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STawhich exhibited biochemical homology to STapurified by more complicated procedures. © 1985.
Saeed, A. M. K., & Greenberg, R. N. (1985). Preparative purification of Escherichia coli heat-stable enterotoxin. Analytical Biochemistry, 151(2), 431–437. https://doi.org/10.1016/0003-2697(85)90200-3