Preparative purification of Plasmodium falciparum circumsporozoite protein synthetic polypeptides by displacement chromatography

  • Viscomi G
  • Ziggiotti A
  • Verdini A
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Abstract

Displacement chromatography was used for the preparative purification of a synthetic polypeptide that is a promising malaria vaccine. It was prepared by solid-phase synthesis and contains two important epitopes of circumsporozoite (CS) protein of Plasmodium falciparum sporozoite. With apparatus typically employed in analytical high-performance liquid chromatography (HPLC) and on a 250 x 4.6 mm I.D. reversed-phase column, up to 50 mg of crude polypeptide were purified in a single run and with a yield higher than 95%. The results demonstrate that displacement chromatography is suitable for the isolation of several milligrams of a pure polypeptide from a complex mixture that is difficult to separate even by analytical HPLC. In such a preparative application, displacement appears to be superior to elution chromatography as used traditionally. © 1989.

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Authors

  • Giuseppe C. Viscomi

  • Alessandra Ziggiotti

  • Antonio S. Verdini

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