Preparative purification of Plasmodium falciparum circumsporozoite protein synthetic polypeptides by displacement chromatography

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Abstract

Displacement chromatography was used for the preparative purification of a synthetic polypeptide that is a promising malaria vaccine. It was prepared by solid-phase synthesis and contains two important epitopes of circumsporozoite (CS) protein of Plasmodium falciparum sporozoite. With apparatus typically employed in analytical high-performance liquid chromatography (HPLC) and on a 250 x 4.6 mm I.D. reversed-phase column, up to 50 mg of crude polypeptide were purified in a single run and with a yield higher than 95%. The results demonstrate that displacement chromatography is suitable for the isolation of several milligrams of a pure polypeptide from a complex mixture that is difficult to separate even by analytical HPLC. In such a preparative application, displacement appears to be superior to elution chromatography as used traditionally. © 1989.

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Viscomi, G. C., Ziggiotti, A., & Verdini, A. S. (1989). Preparative purification of Plasmodium falciparum circumsporozoite protein synthetic polypeptides by displacement chromatography. Journal of Chromatography A, 482(1), 99–106. https://doi.org/10.1016/S0021-9673(01)93210-8

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