Preparative separation of polar unstable compounds (catecholamines) from a synthetic mixture by high-speed counter-current chromatography

  • Weisz A
  • Markey S
  • Ito Y
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Abstract

When a test mixt. of norepinephrine [51-41-2] and dopamine [51-61-6]
was subjected to high-speed counter-current chromatog. on a multilayered
coil planet centrifuge, 2 major peaks (fractions 62-81 and 90-120)
were well resolved and eluted within 2 h. In test runs, 97% of norepinephrine
and 90% of dopamine applied to counter-current chromatog. were recovered
in combined fractions, as detd. by HPLC with electrochem. detection.
Thus, rapid and complete sepn. of the 2 catecholamines from a crude
mixt. shows that the high-speed counter-current chromatog. technique
has a great potential for use. [on SciFinder(R)]

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