Present chromatographic methods of fractionating DNA

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Abstract

Ion-exchange resins and inorganic gels have only limited application in the fractionation of DNA. Although DEAE-cellulose has been used extensively, ECTEO-LA-cellulose has promise in the fractionation of DNA. Carboxymethylcellulose or nitrocellulose have limited capabilities as regards DNA fractionation. Gel filtration techniques employing agar gels, DEAE-Sephadex and DEAE-dextran are of more value in the fractionation of s-RNA and polynucleotides than for highly polymerised DNA. Amongst the kieselguhr columns, the methylated serum albumin-kieselguhr column found wide applicability and gives reproducible profiles. An Amberlite IR-120 Al3+column also gives interesting results with various potential applications. Alternate methods effecting finer resolution are still a pressing need. © 1970.

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Kothari, R. M. (1970). Present chromatographic methods of fractionating DNA. Chromatographic Reviews, 12(2), 127–155. https://doi.org/10.1016/0009-5907(70)80008-4

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