Present chromatographic methods of fractionating DNA

  • Kothari R
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Abstract

Ion-exchange resins and inorganic gels have only limited application in the fractionation of DNA. Although DEAE-cellulose has been used extensively, ECTEO-LA-cellulose has promise in the fractionation of DNA. Carboxymethylcellulose or nitrocellulose have limited capabilities as regards DNA fractionation. Gel filtration techniques employing agar gels, DEAE-Sephadex and DEAE-dextran are of more value in the fractionation of s-RNA and polynucleotides than for highly polymerised DNA. Amongst the kieselguhr columns, the methylated serum albumin-kieselguhr column found wide applicability and gives reproducible profiles. An Amberlite IR-120 Al3+column also gives interesting results with various potential applications. Alternate methods effecting finer resolution are still a pressing need. © 1970.

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Authors

  • R. M. Kothari

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