Preservation of Neisseria meningitidis group B by freeze-drying

  • Moreira T
  • Iglesias E
  • Delgado H
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Abstract

Neisseria meningitidis serogroup B strain B4P115 was freeze-dried using 6 different preservation media. It was observed that a high proportion of sucrose in the freeze-drying medium increases the survival after process but that sodium glutamate was injurious. The influence of two culturing methods before freeze-drying and the behaviour of the lyophilized strain at different storage temperatures was also studied. © 1995.

Author-supplied keywords

  • Freeze-drying
  • Neisseria meningitidis
  • Storage temperature

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Authors

  • Tomás Moreira

  • Elsie Iglesias

  • Herminia Delgado

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