Neisseria meningitidis serogroup B strain B4P115 was freeze-dried using 6 different preservation media. It was observed that a high proportion of sucrose in the freeze-drying medium increases the survival after process but that sodium glutamate was injurious. The influence of two culturing methods before freeze-drying and the behaviour of the lyophilized strain at different storage temperatures was also studied. © 1995.
Moreira, T., Iglesias, E., & Delgado, H. (1995). Preservation of Neisseria meningitidis group B by freeze-drying. Journal of Microbiological Methods, 23(3), 343–346. https://doi.org/10.1016/0167-7012(95)00050-U