Prevalence of β-exotoxin, diarrhoeal toxin and specific δ-endotoxin in natural isolates of Bacillus thuringiensis

  • Perani M
  • Bishop A
  • Vaid A
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Abstract

Of newly isolated colonies with the appearance of Bacillus thuringiensis, 47.5% were found to produce the parasporal crystals characteristic of this species. These positive isolates were screened using the polymerase chain reaction for their possession of a gene encoding a specific protoxin type, CryIB. Strains with and without this gene were screened for their ability to produce β-exotoxin and Bacillus cereus-type enterotoxin. It was found that 35% of the isolates possessed the cryIB gene; of these 83% also produced enterotoxin and 58% produced β-exotoxin. No statistical significance was found for linkage between any of these characteristics. The probability, therefore, of isolating a strain of B. thuringiensis which specifically possessed the cryIB gene but did not produce either of the other, undesired, toxins, from the soil sample used, was 1.2%.

Author-supplied keywords

  • Bacillus cereus-type enterotoxin
  • Bacillus thuringiensis
  • β-exotoxin
  • δ-endotoxin

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Authors

  • Michela Perani

  • Alistair H. Bishop

  • Alka Vaid

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