Prevention of lethal endotoxemia in actinomycin D-sensitized mice by incubation of Salmonella minnesota R595 lipopolysaccharide with monoclonal antibodies to R595

  • Appelmelk B
  • Verweij-van Vught A
  • Brade H
 et al. 
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Abstract

Murine monoclonal antibodies reacting with lipopolysaccharide (LPS) of Salmonella minnesota strain R595 (Re chemotype) were prepared, and tested for their ability to protect actinomycin D-sensitized mice against lethal endotoxemia. Protection was found with some antibodies up to a 90-fold increase in LD50, whereas others exhibited no protection. The various protective antibodies did not all bind to the same epitope. The same applied for non-protective clones. Protective and non-protective clones could not be discriminated by ELISA. One protective monoclonal antibody (clone 20) was specific for ketodeoxyoctonate, a structural element common to various LPS. These findings show that the involvement of lipid A in the binding site of monoclonal antibodies is no prerequisite for protection. © 1988.

Author-supplied keywords

  • lipopolysaccharide
  • monoclonal antibodies
  • neutralization

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Authors

  • B. J. Appelmelk

  • A. M J J Verweij-van Vught

  • H. Brade

  • J. J. Maaskant

  • W. F. Schouten

  • L. G. Thijs

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