Primary structure of the major glycans of the N-acetyllactosamine type derived from the human immunoglobulins M from two patients with Waldenström's macroglobulinemia

  • Cahour A
  • Debeire P
  • Hartmann L
 et al. 
  • 4

    Readers

    Mendeley users who have this article in their library.
  • 23

    Citations

    Citations of this article.

Abstract

The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N-acetyllactosamine-type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high-voltage paper electrophoresis. The primary structure of the major compounds was elucidated on the basis of carbohydrate analysis, methylation analysis, including mass-spectrometry, and 500 MHz1H-NMR spectroscopy. For both patients, this appeared to be a monosialyl monofucosyl biantennary structure; the compounds differed by the presence of an intersecting N-acetylglucosamine residue. © 1984.

Author-supplied keywords

  • 500 MHz1H-NMR spectroscopy
  • Immunoglobulin M
  • N-Glycosidic glycan
  • Waldenström's macroglobulinemia

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • A. Cahour

  • P. Debeire

  • L. Hartmann

  • J. Montreuil

  • H. Van Halbeek

  • J. F.G. Vliegenthart

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free