The primary structure of wheat germ tRNAArg - the substrate for arginyl-tRNAArg: protein transferase

Abstract

Besides its major role in protein synthesis, wheat germ arginyl-tRNAArg can serve as an amino acid donor in an enzymatic reaction to bovine serum albumin catalysed by the enzyme arginyl-tRNAArg: protein transferase. The nucleotide sequence of the tRNAArg involved in this reaction was determined to be: pG-A-C-U-C-C-G-U-m1G-m2G-C-C-C-A-A-D-Gm-G-A-X-A-A-G-G-C-m22G-C-U-G-G-U-Cm-U-I-C-G-m2A-A-A-C-C-A-G-A-G-A-D-U-m5C-U-G-G-G-T--C We suggest that the decapentanucleotide 5′-G-U-Pu-m2G-C-N-C-A-A-D-Gm-G-A-X-A-3′, localized in the D-region, interacts specifically with the protein transferase. © 1986 Société de Chimie biologique / Editions Scientifiques Elsevier, Paris.