A hybrid protein between staphylococcal protein A and human lymphotoxin, ALT, was produced in Escherichia coli by expression of a recombinant plasmid containing the respective genes. IgG-binding activity of ALT was confirmed by Western blotting analysis and by affinity purification with IgG-Sepharose column chromatography. The purified ALT had cytotoxicity on mouse L-929 cells and its specific activity was approximately 3.5-5.0 × 106 U mg-1. ALT was partially degraded by a protease including in the E. coli lysate or trypsin and was converted to lymphotoxin which lacks the NH2-terminal 19 residues but possesses higher cytotoxic activity than ALT. © 1993.
Wada, M., Kasumimoto, T., Miki, T., Osada, H., & Kato, S. (1990). Production of a protein A - lymphotoxin hybrid protein for cancer-targeted therapy. Journal of Biotechnology, 13(4), 325–334. https://doi.org/10.1016/0168-1656(90)90080-U