Production of a protein A - lymphotoxin hybrid protein for cancer-targeted therapy

  • Wada M
  • Kasumimoto T
  • Miki T
 et al. 
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A hybrid protein between staphylococcal protein A and human lymphotoxin, ALT, was produced in Escherichia coli by expression of a recombinant plasmid containing the respective genes. IgG-binding activity of ALT was confirmed by Western blotting analysis and by affinity purification with IgG-Sepharose column chromatography. The purified ALT had cytotoxicity on mouse L-929 cells and its specific activity was approximately 3.5-5.0 × 106U mg-1. ALT was partially degraded by a protease including in the E. coli lysate or trypsin and was converted to lymphotoxin which lacks the NH2-terminal 19 residues but possesses higher cytotoxic activity than ALT. © 1993.

Author-supplied keywords

  • Anti-tumor drug
  • Hybrid protein
  • Lymphotoxin
  • Staphylococcal protein A

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