Prolactin, epidermal growth factor or transforming growth factor-α activate a mammary cell-specific enhancer in mouse mammary tumor virus-long terminal repeat

  • Haraguchi S
  • Good R
  • Engelman R
 et al. 
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Abstract

Mammary specific expression of elevated levels of mouse mammary tumor virus (MMTV) contributes to mammary carcinogenesis. Mechanisms which regulate provirus expression have not been completely defined. Using a MMTV-long repeat terminal (MMTV-LRT) directed chloramphenicol-acetyltransferase (CAT) reporter gene system and a human breast cancer cell line T47D, we demonstrate that prolactin (PRL), epidermal growth factor (EGF), or transforming growth factor-α (TGF-α) act on a mammary cell-specific enhancer at the extreme 5' end of the MMTV-LTR involving sequences - 1094 through - 858. PRL and either EGF or TGF-α exert concerted roles in this activation of these sequences. In contrast, using a plasmid construct lacking this mammary cell-specific enhancer, EGF or TGF-α, but not PRL, act synergistically with progesterone to induce CAT activity, indicating that the action of PRL on regulatory elements of the MMTV-LTR is restricted to this mammary cell-specific enhancer involving sequences - 1094 through - 858. A mobility shift assay was used to demonstrate that PRL, EGF or TGF-α induce nuclear factors (MP4, MAF, and MGF) which bind directly to this mammary cell-specific enhancer element.

Author-supplied keywords

  • Epidermal growth factor
  • Mammary cell-specific enhancer
  • Mouse mammary tumor virus long terminal repeat
  • Prolactin
  • Transforming growth factor-α

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Authors

  • S. Haraguchi

  • R. A. Good

  • R. W. Engelman

  • S. Greene

  • N. K. Day

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