When active chromatin is released as a Mg-soluble fraction following digestion of nuclei with DNAase II, a concomitant release of HMG proteins, and hnRNP particles occurs. Release of HMG 14 and 17 is dependent on active chromatin release, whereas HMG 1 and 2, and hnRNP particles are released in an independent process. The Mg-soluble fraction comprises a heterogeneous mixture of particles of less compact conformation than normal nucleosomes, and prone to protein-induced aggregation. Histone H1, and HMG 14 and 17 appear to be associated with these particles in a reversible manner, whereas HMG 1 and 2 are unbound. © 1981.
CITATION STYLE
Billett, M. A. (1981). Protein distribution amongst chromatin particles following DNAase II digestion. Cell Biology International Reports, 5(1), 55–58. https://doi.org/10.1016/0309-1651(81)90157-0
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