Purification of labelled antibodies by hydrophobic interaction chromatography

4Citations
Citations of this article
1Readers
Mendeley users who have this article in their library.
Get full text

Abstract

In order to improve the sensitivity of immunometric assays, a chromatographic technique was developed that virtually eliminates components causing non-specific background. Labelled antibodies were applied to a phenyl-Sepharose column in physiological buffer. When labelled anitbodies were purified by this technique, the non-specific background of various time-resolved immunofluorometric assays was reduced 3- to 10-fold and was very close to the instrument background. The assay sensitivity was simultaneously increased by a factor of 2 to 16. This purification method might be used to improve the results of immunometric assays in general. © 1989.

Cite

CITATION STYLE

APA

Alfthan, H., & Stenman, U. H. (1989). Purification of labelled antibodies by hydrophobic interaction chromatography. Journal of Chromatography A, 470(2), 385–389. https://doi.org/10.1016/S0021-9673(01)83566-4

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free