Purification of labelled antibodies by hydrophobic interaction chromatography

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In order to improve the sensitivity of immunometric assays, a chromatographic technique was developed that virtually eliminates components causing non-specific background. Labelled antibodies were applied to a phenyl-Sepharose column in physiological buffer. When labelled anitbodies were purified by this technique, the non-specific background of various time-resolved immunofluorometric assays was reduced 3- to 10-fold and was very close to the instrument background. The assay sensitivity was simultaneously increased by a factor of 2 to 16. This purification method might be used to improve the results of immunometric assays in general. © 1989.




Alfthan, H., & Stenman, U. H. (1989). Purification of labelled antibodies by hydrophobic interaction chromatography. Journal of Chromatography A, 470(2), 385–389. https://doi.org/10.1016/S0021-9673(01)83566-4

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