Purification of a novel DBI processing product, DBI39-75, and characterization of its binding site in rat brain

  • Slobodyansky E
  • Antkiewicz-Michaluk L
  • Martin B
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Abstract

This report describes the purification and characterization from rat brain of DBI39-75, a novel, biologically active processing product of diazepam binding inhibitor (DBI). We have shown that DBI39-75in nM concentrations stimulates pregnenolone synthesis in mitochondria of rat brain. Using cross-linking of125I-DBI39-75in steroidogenically active concentrations we have demonstrated for the first time that the DBI fragment has a specific high affinity binding site in rat brain. Displacement of [3H]PK 11195 and [3H]Ro5-4864 by DBI39-75indicates more complex interaction than the competitive inhibition. Collectively, the data suggest that the function of DBI39-75is mediated through a mitochondrial receptor complex which includes binding sites for PK 11195 and Ro5-4864. © 1994.

Author-supplied keywords

  • Cross-linking
  • DBI39-75
  • Neuropeptide
  • PK 11195
  • Pregnenolone
  • Ro5-4864

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Authors

  • Elizabeth Slobodyansky

  • Lucyna Antkiewicz-Michaluk

  • Brian Martin

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