Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography

  • Chen F
  • Lu H
  • Jiang Y
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Abstract

High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run. From 160 mg of the crude sample containing 22.0% paeoniflorin, 33.2 mg of paeoniflorin was yielded at 98.2% purity as determined by HPLC analysis. The recovery of paeoniflorin was 94.3%. © 2004 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Counter-current chromatography
  • Paeonia lactiflora
  • Paeoniflorin
  • Pharmaceutical analysis
  • Plant materials
  • Preparative chromatography

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