Purification of a UTP: d-Glucose-1-phosphate uridylyl-transferase from Golgi apparatus of cat liver by affinity chromatography on UTP-agarose

  • Azzar G
  • Persat F
  • Degiuli A
 et al. 
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Abstract

UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characteristics, UDP-glucose pyrophosphorylase from Golgi apparatus is different from cytosolic enzyme. © 1984.

Author-supplied keywords

  • Golgi apparatus
  • UDP-glucose pyrophosphorylase
  • UTP-agarose
  • affinity chromatography

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Authors

  • G. Azzar

  • F. Persat

  • A. Degiuli

  • R. Got

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