A quantitative collagen film collagenase assay for large numbers of samples

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Abstract

A simple, rapid, sensitive procedure for determining the collagenase activity of a large number of samples is presented. The method is performed in plastic plates of 96 flat-bottom microwells in which14C-labeled collagen is dried to a film from thermally reconstituted fibrils. The collagenase assay consists of adding enzyme samples to the wells, incubating them for relatively short times, and removing the fluid for scintillation counting. The method is quantitative, sensitive (detects at least 10 ng bacterial collagenase, 1.6 μg purified vertebrate collagenase), and precise (SD = 4% for n = 3). © 1980.

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Johnson-Wint, B. (1980). A quantitative collagen film collagenase assay for large numbers of samples. Analytical Biochemistry, 104(1), 175–181. https://doi.org/10.1016/0003-2697(80)90295-X

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