The use of random amplified polymorphic DNA to identify pathotype and detect variation in Pseudocercosporella herpotrichoides

  • Nicholson P
  • Rezanoor H
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Abstract

We have used a PCR technique, based upon the random amplification of polymorphic DNA (RAPD) using 10-mer oligonucleotide primers to investigate variability between 23 isolates of Pseudocercosporella herpotrichoides from the U.K. and a range of other locations. Individual isolates were clearly distinguished using analysis of complete amplification profiles (haplotypes), or of major bands only, for the 10 primers tested. Cluster analysis of haplotypes, or of major bands only, clearly distinguished two groups, one of which related to isolates pathogenic on rye (R-type). In addition, several primers amplified bands specific to isolates pathogenic on Agropyron repens (C-types) and cluster analysis of the major amplification products in profiles differentiated the isolates into three groups relating to pathogenicity. Within these groups variation in the RAPD profiles did not appear to be related to origin. Further uses and developments of RAPD analysis are discussed. © 1994, British Mycological Society. All rights reserved.

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Authors

  • P. Nicholson

  • H. N. Rezanoor

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