Use of a rapid DNA sequencing system to demonstrate the induction of frameshift mutations by bleomycin

  • Demopoulos N
  • Wayne Davies R
  • Scazzocchio C
  • 3

    Readers

    Mendeley users who have this article in their library.
  • 7

    Citations

    Citations of this article.

Abstract

The rapid DNA sequencing system based on the single-stranded bacteriophage M13 and the chain-terminator method has been used to look directly for mutational alterations. A small DNA fragment that primes DNA synthesis through the N-terminal 200 base pairs of the β-galactosidase gene was prepared, and used to detect changes in base sequence among phages that give white plaques after treatment of the host cells with bleomycin. Bleomycin treatment of E. coli in which M13 mp2 was growing gave an increase in white plaque frequency. DNA sequence analysis of phage from 7 independent mutant plaques showed them all to have a frameshift mutation. © 1982.

Author-supplied keywords

  • Bleomycin
  • Carcinogenesis
  • DNA sequencing M13
  • Frameshift
  • Mutagenesis screen

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • N. Demopoulos

  • R. Wayne Davies

  • C. Scazzocchio

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free