Rapid isolation of insect ribosomal subunits by ethanol-magnesium precipitation

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Abstract

Insect ribosomal subunits may be isolated from gradient fractions in 10-15 min by precipitation with 0.2 vol ethanol in the presence of 10 mM Mg2+. The yield is approximately 90%, while protein synthetic activity, response to exogenous template and sedimentation behavior of the ribosomes are unaffected by this treatment. © 1971.

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Kaulenas, M. S. (1971). Rapid isolation of insect ribosomal subunits by ethanol-magnesium precipitation. Analytical Biochemistry, 41(1), 126–131. https://doi.org/10.1016/0003-2697(71)90197-7

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