A rapid mixing-photocrosslinking technique to study the dynamics of nucleic acid-protein interactions

  • Wu C
  • Hillel Z
  • Park C
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Abstract

A rapid mixing-photocrosslinking technique has been developed to investigate the kinetics of protein-nucleic acid interactions. With this technique, binding of nucleic acid to protein is first synchronized by rapid mixing in a stopped-flow apparatus. The intermediates formed at different stages of the binding process are then "frozen" by photocrosslinking with a 10-μs uv light pulse at various times after mixing. By analyzing structural changes of these intermediates as a function of time, one can obtain the information concerning the dynamic aspects of the interaction. This technique may also be applied to other macromolecular interactions in biological systems. © 1983.

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Authors

  • Cheng Wen Wu

  • Zaharia Hillel

  • Chan Suk Park

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