Rat urinary kallikrein was separated by high-performance liquid chromatography (HPLC) using an ion-exchange or gel-permeation column. Kallikrein activity was monitored continuously with peptidase or esterase activity using a post-reactor system directly adapted to HPLC. A PTFE helically coiled tube served as the enzyme reactor vessel. Four and three peaks with peptidase and esterase activity, respectively, were detected on application of normal rat urine. © 1983.
Funae, Y., Akiyama, H., Imaoka, S., Takaoka, M., & Morimoto, S. (1983). Rapid separation and measurement of rat urinary kallikrein by high-performance liquid chromatography with a continuous flow enzyme detector. Journal of Chromatography A, 264(C), 249–257. https://doi.org/10.1016/S0021-9673(01)95028-9