A reaction detector based on bromine consumption for liquid chromatography is presented. Bromine is electrochemically generated on-line in a separate flow, which combines in a post-column mode with the column effluent. After the passage of a single-bead-string reactor, with 10-20 sec in residence time, the bromine concentration is monitored by reductive amperometry. Bromine-reactive substances will cause a decrease in amperometric response in proportion to concentration, and an indirect quantification is thus achieved. Detection capabilities are illustrated for methyl-, ethyl- and propylamine, reduced and oxidized glutathione and Iysozyme and cytochrome c. Depending on reaction conditions, linear dynamic ranges are between one and three orders of magnitude. Representative detection limits are 0.5-10 picomoles with use of conventional liquid-chromatographic equipment. © 1985.
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