Reaction detector for liquid chromatography with electrochemical generation and detection of excess bromine

  • Isaksson K
  • Lindquist J
  • Lundström K
  • 1


    Mendeley users who have this article in their library.
  • 20


    Citations of this article.


A reaction detector based on bromine consumption for liquid chromatography is presented. Bromine is electrochemically generated on-line in a separate flow, which combines in a post-column mode with the column effluent. After the passage of a single-bead-string reactor, with 10-20 sec in residence time, the bromine concentration is monitored by reductive amperometry. Bromine-reactive substances will cause a decrease in amperometric response in proportion to concentration, and an indirect quantification is thus achieved. Detection capabilities are illustrated for methyl-, ethyl- and propylamine, reduced and oxidized glutathione and Iysozyme and cytochrome c. Depending on reaction conditions, linear dynamic ranges are between one and three orders of magnitude. Representative detection limits are 0.5-10 picomoles with use of conventional liquid-chromatographic equipment. © 1985.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Karin Isaksson

  • Jörgen Lindquist

  • Kent Lundström

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free