On the reactions of fluorescamine with chromosomal proteins

10Citations
Citations of this article
4Readers
Mendeley users who have this article in their library.
Get full text

Abstract

α- and ε-amino groups of basic chromosomal proteins (protamines and histones) are available for the reaction with fluorescamine. The extent of the reaction depends on the respective pKavalues but linear relationships between the protein concentration and fluorescence were found at all pH values tested (5.5-10.5). Upon interaction with DNA, the pKavalues of protein amino groups are raised to an extent which excludes them from the fluorescamine reaction under quasiphysiological conditions. Protein displacements caused by dissociating agents may conveniently be followed kinetically and can be quantified by appropriate applications of fluorescamine. © 1979 Academic Press, Inc.

Cite

CITATION STYLE

APA

Bode, J. (1979). On the reactions of fluorescamine with chromosomal proteins. Analytical Biochemistry, 99(2), 274–280. https://doi.org/10.1016/S0003-2697(79)80006-8

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free