Recombinant (2→3)-α-sialyltransferase immobilized on nickel-Agarose for preparative synthesis of sialyl Lewisxand Lewisaprecursor oligosaccharides

16Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The specificity of recombinant (2→3)-α-sialyltransferase (ST3Gal-III), expressed in baculovirus-infected insect cells, has been determined with various oligosaccharide acceptors and sugar-nucleotide donors using a fluorescence based assay. Recombinant ST3Gal-III tagged with a polyhistidine tail was immobilized on Ni2+-NTA-Agarose as an active enzyme for use in the synthesis of three sialylated oligosaccharides: (i) the divalent molecule [α-Neu5Ac-(2→3)-D-Galp-(1→4)-β-D-GlcpNAc-O-CH2]2-C-(CH2OBn)2(12); (ii) the dansylated derivative, α-Neu5Ac-(2→3)-D-Galp-(1→3)-β-D-GlcpNAc-O-(CH2)6-NH-dansyl and; (iii) the tetrasacharide α-Neu5Ac-(2→3)-β-D-Galp-(1→4)-β-D-GlcpNAc- (1→2)-α-D-Manp-O-CH3. Compound 12 was itself prepared from the divalent N-acetyllactosamine molecule built on pentaerythritol by a chemo-enzymatic route. © 2003 Elsevier Science Ltd. All rights reserved.

Cite

CITATION STYLE

APA

Ivannikova, T., Bintein, F., Malleron, A., Juliant, S., Cerutti, M., Harduin-Lepers, A., … Lubineau, A. (2003). Recombinant (2→3)-α-sialyltransferase immobilized on nickel-Agarose for preparative synthesis of sialyl Lewisxand Lewisaprecursor oligosaccharides. Carbohydrate Research, 338(11), 1153–1161. https://doi.org/10.1016/S0008-6215(03)00130-7

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free