Regeneration of denatured polyphenol oxidase in Toona sinensis (A.Juss.) Roam

Citations of this article
Mendeley users who have this article in their library.
Get full text


The regeneration of polyphenol oxidase (PPO) from leaves of Toona sinensis (A.Juss.) Roam (TS), denatured by boiling with 0.4% (w/v) sodium dodecyl sulfate (SDS) and 1% (v/v) β-mercaptoethanol, and was studied with SDS-polyacrylamide gel electrophoresis (PAGE) and the gel-activity-stained assay. The optimal regenerating conditions for the denatured TS-PPO were as follows: extracting with buffer of 150 mM Na-Pi, pH 7.2; regenerating with buffer of 50 mM Tris-HCl, pH 7.2, 2% Triton X-100 for 45 min. In addition, the PPO regeneration was promoted by the addition of Cu2+in the recovery buffer. The regenerating activity of the denatured-PPO was reduced in samples extracted with buffers containing polyvinylpyrrolidone (PVP), polyvinylpolypyrrolidone (PVPP) or Triton X-100. © 2007 Elsevier Ltd. All rights reserved.




Feng, W., Wang, M., Cao, J., Sun, J., & Jiang, W. (2007). Regeneration of denatured polyphenol oxidase in Toona sinensis (A.Juss.) Roam. Process Biochemistry, 42(7), 1155–1159.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free