1. 1. Tritium labelling of purified major and minor phosphoproteins from hen egg phosvitin was carried out by reductive methylation. 2. 2. Standard additions of the labelled phosphoproteins were made to fresh egg yolk samples and the phosvitin then extracted by the Joubert-Cook procedure. Distribution of radioactivity was measured at each step of the extraction. 3. 3. The extraction method isolates half of the phosvitin in hen egg yolk. Phosvitin structure accounts for a fifth of the total yolk phosphorous content. Mol ratio of phosvitin major phosphoprotein to minor phosphoprotein is 2:1 in extracted phosvitin, whole egg yolk, and is likely to be similar in vitellogenin as well. © 1980.
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