This chapter describes the status of recombinant inbred (RI) strains as model systems for identifying novel genes involved in the hematopoietic disease, such as ecotropic viral integration site-1 (Evi-1). The chapter discusses Evi-1 and its relationship to other members of the zinc-finger protein family. A common viral integration site, Evi-1 was identified in myeloid tumors of AKXD-23 recombinant inbred (RI) mice. Evi-1 identifies a novel locus that encodes a zinc-finger protein. DNAs from cell lines established from myeloid tumors that arose in virus-infected NFS/N or NFS/N hybrid mice were screened for rearrangements in the Evi-1 locus. Results indicated that Myb and Evi-1 gene products cooperate in inducing the myeloid disease. The AKXD RI strains were derived by crossing mice from two inbred strains that differed significantly in lymphoma incidence: AKR/J and DBA/2J. The low level of virus expression in DBA/2J mice is an important factor contributing to the low tumor incidence in this strain. © 1990 Academic Press Inc.
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