In search of a planar and uniform model membrane presenting a wide interaction-surface for the study of photobiological processes, we have succeeded in depositing lipid bilayer on a single face of a porous disc. Using a method that combines the Langmuir-Blodgett dipping and the touching procedures, a symmetrical or asymmetrical bilayer membrane has been obtained. The illumination of rhodopsin-containing membranes appears to increase the permeability to K+, Na+, and Ca2+with a high selectivity for Ca2+. The bilayer adsorption on the porous disc is discussed and an attempt is made to explain the failure of the dipping method in our experimental conditions for preparing an artificial membrane. A comparative look at model membranes is also presented. © 1981.
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