A method using saturation transfer EPR (ST-EPR) is shown to be feasible for detecting EPR signal of radiation-induced defects in biocarbonates containing large amount of Mn2+. The ST-EPR measurements conducted at room temperature on fish fossil of Brazilian origin, enabled the identification of CO2-and SO3-radical ions, by partially suppressing the intense signal from Mn2+when the signal are detected 90° out of phase with magnetic field modulating signal and at high microwave power (50 mW). Using these signals the age of fish fossil is estimated to be (36±5) Ma. © 2003 Elsevier Science Ltd. All rights reserved.
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