Secretoneurin-induced in vitro chemotaxis of human monocytes is inhibited by pertussis toxin and an inhibitor of protein kinase C

  • Schratzberger P
  • Wöll E
  • Reinisch N
 et al. 
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The sensory neuropeptide secretoneurin (SN) triggers chemotactic migration of monocytes. We have investigated the possibility that SN, like other chemoattractants such as formyl-Met-Leu-Phe and chemokines, might stimulate migration of monocytes by G protein and protein kinase C (PKC) activation and induce Ca(i)2+release. We report that preincubation of monocytes with pertussis toxin inhibited SN chemotaxis. Staurosporine, an inhibitor of PKC, significantly decreased SN-induced chemotaxis of monocytes, suggesting that PKC may be involved in the signaling. Tyrphostin-23, which inhibits tyrosin kinase, did not affect SN-induced chemotaxis of monocytes. This suggests that SN uses a signaling mechanism that is coupled to pertussis toxin-sensitive G proteins. Involvement of phospholipase Cβ as a result of PKC activation is suggested by a SN-induced increase of intracellular Ca2+concentration in monocytes.

Author-supplied keywords

  • Inflammation
  • Neuroimmunomodulation
  • Phagocytes
  • Protein kinase C
  • Receptors
  • Secretogranin II

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  • Peter Schratzberger

  • Ewald Wöll

  • Norbert Reinisch

  • Christian M. Kähler

  • Christian J. Wiedermann

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