Secretoneurin-induced in vitro chemotaxis of human monocytes is inhibited by pertussis toxin and an inhibitor of protein kinase C

29Citations
Citations of this article
3Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The sensory neuropeptide secretoneurin (SN) triggers chemotactic migration of monocytes. We have investigated the possibility that SN, like other chemoattractants such as formyl-Met-Leu-Phe and chemokines, might stimulate migration of monocytes by G protein and protein kinase C (PKC) activation and induce Ca(i)2+release. We report that preincubation of monocytes with pertussis toxin inhibited SN chemotaxis. Staurosporine, an inhibitor of PKC, significantly decreased SN-induced chemotaxis of monocytes, suggesting that PKC may be involved in the signaling. Tyrphostin-23, which inhibits tyrosin kinase, did not affect SN-induced chemotaxis of monocytes. This suggests that SN uses a signaling mechanism that is coupled to pertussis toxin-sensitive G proteins. Involvement of phospholipase Cβ as a result of PKC activation is suggested by a SN-induced increase of intracellular Ca2+concentration in monocytes.

Cite

CITATION STYLE

APA

Schratzberger, P., Wöll, E., Reinisch, N., Kähler, C. M., & Wiedermann, C. J. (1996). Secretoneurin-induced in vitro chemotaxis of human monocytes is inhibited by pertussis toxin and an inhibitor of protein kinase C. Neuroscience Letters, 214(2–3), 208–210. https://doi.org/10.1016/0304-3940(96)12947-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free