Selected-ion monitoring gas chromatographic-mass spectrometric analysis of catecholamines: enhancement of sensitivity by a simple clean-up step on Sephadex G-10

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Abstract

The sensitivity of catecholamines using selected-ion monitoring gas chromatography-mass spectrometry (SIM GC-MS) has been enhanced by employing a simple clean-up step using Sephadex G-10 columns. This procedure allows for the detection of extremely small amounts (1-10 pg) of biogenic amines (e.g., epinine) which corresponds to a 25-fold increase in detection limits compared to electron ionization GC-MS or high-performance liquid chromatography with electrochemical detection (HPLC-ED). The SIM GC-MS assay was used to monitor the dopamine-β-hydroxylase-mediated conversion of epinine d6 to epinephrine d6 in rat hypothalamus and brainstem in vitro and the results were compared to those obtained by HPLC-ED. © 1988.

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Scriba, G. K. E., Borchardt, R. T., Zirrolli, J. A., & Fennessey, P. V. (1988). Selected-ion monitoring gas chromatographic-mass spectrometric analysis of catecholamines: enhancement of sensitivity by a simple clean-up step on Sephadex G-10. Journal of Chromatography B: Biomedical Sciences and Applications, 433(C), 31–40. https://doi.org/10.1016/S0378-4347(00)80582-4

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