Self cleavage of C8-histamino-r(UpA) promoted by ZnCl2: Mechanistic studies on a designed ribonuclease mimic

  • Prakash T
  • Kunte S
  • Ganesh K
  • 3


    Mendeley users who have this article in their library.
  • 5


    Citations of this article.


Studies on self cleavage of dinucleotides (2-5) containing conjugated imidazole and ethylenediamine chains indicated that the imidazole conjugated ribodinucleotide 4 hydrolyzed in presence of ZnCl2, whereas other dinucleotides 2, 3 and 5 were inert. An acceleration of 10-15 times in rate of hydrolysis of 4 was observed as compared to the unmodified dinucleotide 6. The product analysis by HPLC suggested that the reaction involves cleavage of P-O5′ bond with the formation of C8-histamino dA 9 and 2′,3′-cUMP 10 which subsequently hydrolyzed to a mixture of 2′ and 3′ UMP by a slower reaction. The mechanism of hydrolysis of designed model compound 4 is similar to that of the first step of hydrolysis of RNA by RNase A. © 1994.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free