The separation of a series of glucosinolates by reversed-phase ion-pair liquid chromatography is reported. A rapid and adequate separation was achieved on a column of Nucleosil 5 C18, with 0.01 M phosphate buffer at pH 7.0-methanol (3:7) containing 0.005 M tetraheptylammonium bromide as mobile phase. The modifier concentration, the nature of the counter-ion and the pH greatly influence the separation of the glucosinolates from each other and from non-ionic impurities. The isolation of the total glucosinolate fraction was performed by a newly developed ion-exchange chromatographic method. By combination with the separation method described here, acidic and basic conditions (which would lead to decomposition of the glucosinolates) are completely avoided. The proposed method is briefly discussed in relation to methods previously used for the isolation, identification and quantitative determination of glucosinolates. © 1980, All rights reserved.
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