Separation of neutral nitrogen compounds from synthetic crude oils for biological testing

  • Ho C
  • Ma C
  • Clark B
 et al. 
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Abstract

Isolation of neutral N-PAHs (PAH homologues containing one or more ring nitrogens) is achieved in three steps using acid-base extraction, gel filtration on Sephadex LH-20, and adsorption chromatography on silicic acid. Gas chromatographic/mass spectrometric analysis of the neutral N-PAH fractions indicated the following as major components: C1-C3phenylpyrroles, indole, C1-C6indoles, C1-C3phenylindoles, carbazole, C1-C5carbazoles, benzocarbazoles, and C1-C3benzocarbazoles. The neutral N-PAH fractions were subjected to mutagenicity tests using Salmonella typhimurium/microsomal activation systems devised by Ames. The neutral N-PAH fraction of the coal-derived oil had a specific activity more than twice that of the PAH fraction of the same oil, whereas the shale oil neutral N-PAH fraction showed no activity. These results are discussed in the context of previous work with these oils and with some pure neutral N-PAH compounds. © 1980, All rights reserved.

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Authors

  • C. H. Ho

  • C. Y. Ma

  • B. R. Clark

  • M. R. Guerin

  • T. K. Rao

  • J. L. Epler

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