Separation of neutral reducing oligosaccharides derived from glycoproteins by HPLC on a hydroxylated polymeric support

  • Bendiak B
  • Orr J
  • Brockhausen I
 et al. 
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Abstract

A prototype hydroxylated polymeric support, Glyco-PAK N, was employed to separate a number of neutral reducing oligosaccharides derived from various glycoproteins by the hydrazinolysis/re-N-acetylation/mild acid hydrolysis procedure. General features of the separation properties of the matrix are described. A major advantage of the packing over silica-based supports is its stability, a property which is advantageous both in repetitive analytical chromatography and in the preparative isolation and spectroscopic identification of pure compounds, free of column breakdown products. © 1988.

Author-supplied keywords

  • HPLC
  • NMR
  • glycoproteins
  • hydrazinolysis
  • oligosaccharides
  • polymeric column

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Authors

  • Brad Bendiak

  • Jeanne Orr

  • Inka Brockhausen

  • George Vella

  • Charles Phoebe

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