Separation of neutral reducing oligosaccharides derived from glycoproteins by HPLC on a hydroxylated polymeric support

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Abstract

A prototype hydroxylated polymeric support, Glyco-PAK N, was employed to separate a number of neutral reducing oligosaccharides derived from various glycoproteins by the hydrazinolysis/re-N-acetylation/mild acid hydrolysis procedure. General features of the separation properties of the matrix are described. A major advantage of the packing over silica-based supports is its stability, a property which is advantageous both in repetitive analytical chromatography and in the preparative isolation and spectroscopic identification of pure compounds, free of column breakdown products. © 1988.

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Bendiak, B., Orr, J., Brockhausen, I., Vella, G., & Phoebe, C. (1988). Separation of neutral reducing oligosaccharides derived from glycoproteins by HPLC on a hydroxylated polymeric support. Analytical Biochemistry, 175(1), 96–105. https://doi.org/10.1016/0003-2697(88)90366-1

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