Tyrosine hydroxylase, the rate limiting enzyme in the biosynthesis of catecholamine, is a tetramer composed of four subunits of the same molecular mass. A full length cDNA clone encoding tyrosine hydroxylase has been inserted into the SP6 expression system. Translation of the corresponding RNA in Xenopus oocyte results in enzymatic activity, demonstrating that a single gene contains all the necessary genetic information to code for a functional enzyme. The potential of this system in the analysis of posttranslational tyrosine hydroxylase modifications is discussed. © 1986.
Horellou, P., Guibert, B., Leviel, V., & Mallet, J. (1986). A single RNA species injected in Xenopus oocyte directs the synthesis of active tyrosine hydroxylase. FEBS Letters, 205(1), 6–10. https://doi.org/10.1016/0014-5793(86)80854-7