A single RNA species injected in Xenopus oocyte directs the synthesis of active tyrosine hydroxylase

  • Horellou P
  • Guibert B
  • Leviel V
 et al. 
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Abstract

Tyrosine hydroxylase, the rate limiting enzyme in the biosynthesis of catecholamine, is a tetramer composed of four subunits of the same molecular mass. A full length cDNA clone encoding tyrosine hydroxylase has been inserted into the SP6 expression system. Translation of the corresponding RNA in Xenopus oocyte results in enzymatic activity, demonstrating that a single gene contains all the necessary genetic information to code for a functional enzyme. The potential of this system in the analysis of posttranslational tyrosine hydroxylase modifications is discussed. © 1986.

Author-supplied keywords

  • (Xenopus laevis)
  • SP6 polymerase
  • Translation
  • Tyrosine hydroxylase

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Authors

  • Philippe Horellou

  • Bernard Guibert

  • Vincent Leviel

  • Jacques Mallet

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