Site-specific natural isotope fractionation of hydrogen studied by nuclear magnetic resonance has been applied to cellulose. A combination of n.m.r. and m.s. techniques has been used to measure the isotope ratios of cellulose nitrate, glucose pentanitrate, glucose penta-acetate, and ethanol obtained by fermentation. Fractionation effects during enzymic hydrolysis, chemical derivatization, separation, and purification have been investigated. © 1990.
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