Hemolytic activity in the hemolymph of Spirographis spallanzanii was estabilished against Bufo, sheep, calf, rabbit, rat, human A, B, O erythrocytes. Chemico-physical treatments suggest that the hemolytic factor could be a thermo-labile protein whose activity requires calcium ions and which is active over broad pH and temperature ranges. Some of these properties and the sigmoidal curve obtained by plotting the quantity of hemolymph against the percentage of hemolysis, produce some analogies with the mammalian lytic system. © 1981 Pergamon Press Ltd. All rights reserved.
Parrinello, N., & Rindone, D. (1981). Studies on the natural hemolytic system of the annelid worm Spirographis spallanzanii Viviani (Polychaeta). Developmental and Comparative Immunology, 5(1), 33–42. https://doi.org/10.1016/S0145-305X(81)80005-5