Study of intracellular iron distribution in rabbit reticulocytes with normal and inhibited heme synthesis

  • Borová J
  • Poňka P
  • Neuwirt J
  • 1


    Mendeley users who have this article in their library.
  • 82


    Citations of this article.


Iron compartments in which iron accumulates during the inhibited heme synthesis after treatment with isonicotinic acid hydrazide were studied in rabbit reticulocytes. A great accumulation of59Fe radioactivity was found in mitochondria, low molecular weight iron compounds and non-hemoglobin proteins, especially ferritin. In a chase experiment, approximately 50% of the59Fe radioactivity accumulated in mitochondria and low molecular weight iron compounds was re-utilized for the synthesis of hemoglobin. Although some iron is incorporated into ferritin, it apparently is not utilized for heme synthesis. In control reticulocytes, only traces of59Fe radioactivity were detected in mitochondria and only a minute amount of59Fe radioactivity was detected in low molecular weight iron compounds. The release of low molecular weight iron from59Fe-transferrin occured in intact reticulocytes to a larger extent than in the stroma-free hemolysate. An attempt to establish the possible pathway of iron transport inside the reticulocyte was made. It is suggested that an iron-transferrin complexes enters the reticulocyte cytoplasm, and the majority of released iron is taken up by mitochondria for heme synthesis. When protoporphyrin IX is not available, iron accumulates inside the mitochondria. © 1973.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Jitka Borová

  • Přemysl Poňka

  • Jan Neuwirt

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free