Study of steroid-protein binding by a novel "two-tier" column employing cibacron blue F3G-A-Sepharose 4B. I-Sex hormone binding globulin

  • Iqbal M
  • Johnson M
  • 5

    Readers

    Mendeley users who have this article in their library.
  • 74

    Citations

    Citations of this article.

Abstract

Cibracron Blue F3G-A-Sepharose gel was utilized as an affinity matrix for the removal of albumin from plasma prior to gel filtration on Sephadex LH-20. The incorporation of affinity chromatography and gel filtration in the same column allows for direct and simultaneous evaluation of steroid binding to both non-specific and specific binding components in human plasma. The binding parameters of Sex Hormone Binding Globulin, SHBG, are presented. The application of 5α-Dihydrotestosterone-3(O-carboxymethyl) oxime-human serum albumin, DHT-3-CMO-HSA, conjugate used in conjunction with Cibacron Blue F3G-A-Sepharose is discussed together with results on the relative affinity of steroid derivatives pertinent to the affinity purification of SHBG. The usefulness of this affinity matrix in enrichment of steroid haptens and its possible application to purifying antibodies is also discussed. © 1977.

Author-supplied keywords

  • 1,3,5,(10)-Estratriene-3,17β-diol
  • 17β-hydroxy-5α-androstan-3-one
  • 3β, 17β-Dihydroxy-5α-androstane
  • 5α-Dihydrotestoster-one (DHT)
  • Andros-tanediol
  • Estradiol

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • M. Jawed Iqbal

  • Michael W. Johnson

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free